Developing
materials with remote controllability of macroscale
ligand presentation can mimic extracellular matrix (ECM) remodeling
to regulate cellular adhesion in vivo. Herein, we
designed charged mobile nanoligands with superparamagnetic nanomaterials
amine-functionalized and conjugated with polyethylene glycol linker
and negatively charged RGD ligand. We coupled negatively a charged
nanoligand to a positively charged substrate by optimizing electrostatic
interactions to allow reversible planar movement. We demonstrate the
imaging of both macroscale and in situ nanoscale
nanoligand movement by magnetically attracting charged nanoligand
to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem
cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in
situ magnetic attraction of charged nanoligand stimulates
mechanosensing-mediated differentiation of stem cells. This remote
controllability of ECM-mimicking reversible ligand variations is promising
for regulating diverse reparative cellular processes in vivo.
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