The crude extract of the leaves and stalk of Hippocratea obtusifolia (commonly known as gwadayi in Hausa) was sequentially extracted with petroleum ether, chloroform, ethyl acetate and methanol. The extracts were subjected to bioactivity testing against lactation inducement in 24 female guinea pigs. The experimental animals with average weight of 200g were randomly grouped into six; the first five groups A, B, C, D and E were force-fed with feeds mixed with a measured portion of the extract, while the last group was force-fed with food mixed with distilled water only serving as control. The chloroform extract was found to be the only active extract, and this confirms the use of Hippocratea obtusifolia in lactation inducement.
The ethanolic extract of the leaves of Lawsonia inermis (commonly known as lalle in Hausa) was sequentially extracted with petroleum ether, chloroform, ethyl acetate and methanol. The extracts were respectively labeled LI1, LI2, LI3, and LI4, with the ethanolic extract labeled LI, and 12.5% and 25% of the test extracts were tested for repellency against Anopheles gambiae, and the repellent activity was assessed using the human-bait technique. Five volunteers participated in the laboratory tests, one each for the extracts. The chloroform extract labeled LI1-02 was found to be the most active, (100% repellency), while the extract labeled LI1-03 (25%) recorded the least activity (15.4%). It can be concluded that the chloroform extract labeled LI1-02 is responsible for repellent activity of Lawsonia inermis.
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