Present study was carried out to determine the phytochemical constituents and activities of aqueous ethanolic leaf extract from Tapinanthus dodoneifolius grown on Tamarindus indica tree. Results obtained for phytochemical revealed the presence of flavonoids, tannins, saponins, glycosides and alkaloids. Antiulcer activity o ethanolic leaf extract from Tapinanthus dodoneifolius grown on Tamarindus indica tree was tested on wistar rats' stomach which was compared with standard antiulcer drug Omeparazole. Oral administration of aqueous ethanolic leaf extract from Tapin dodoneifolius (500-1500mg/cm 3 ) to Aspirin induced ulcerated wistar rats for one week reduced the incident of ulceration in a dose dependent manner. Acute toxicity study of the extract did not manifest any toxicological signs in rats. Thus the plant relatively safe and it has potential antiulcer activity.
Ethanol extract of Bryophyllum pinnatum (commonly known as 'Shuka halinka' or 'Karan masallachi' in Hausa) (BP1) was partitioned into n-hexane, chloroform, ethyl acetate and aqueous methanol soluble fractions and labeled BP1-01, BP1-02, BP1-03 and BP1-04 respectively. These fractions were subjected to antibacterial testing against respiratory tract pathogenic bacteria. The n-hexane soluble fraction showed activity against the selected microorganism with highest on Staphylococcus aureus (12mm), Klebsiella pneumonia (11mm) and Salmonella typhi (08mm); ethyl acetate soluble fraction showed mild activity against Escherichia coli (06mm), Staphylococcus aureus (07mm) and Salmonella typhi (07mm), at 10mg/ml. The n-hexane soluble fraction was subjected to activity guided column chromatography. This leads to isolation of three bioactive compounds: BP1-01-01, BP1-01-33 and BP1-01-65. The activity of this plant's extracts against the test bacteria coupled with the various phytochemical compounds present in the fractions is a pointer to the plant's potential as a source of drugs that can be used against respiratory tract pathogens.
The leaves of Terminalia catappa were air dried, grounded and soaked with ethanol. The extracts obtained (47.23g, TC1) was fractionated sequentially using aqueous methanol with petroleum ether, chloroform and ethyl acetate respectively. The residue of ethanol extract (marc) was extracted with 5M HCl, basified and extracted with chloroform. These were labeled as TC1-01-TC1-05 for the plant. Each of these fractions was phytochemically screened to detect the class of secondary metabolite present. The fractions obtained from the plant were found to be selectively active against brine shrimp larvae. These fractions were also subjected to antimalaria parasites bioassay. Fractions TC1, TC1-02 and TC1-05 were found to be active against tested organisms, with TC1-02 being the most active. TC1-02 was further subjected to activity guided column chromatography that led to the isolation of two pure compounds TC1-02-1 and TC1-02-64. Compound TC1-02-64 was found to be active against the malaria parasite. This was further purified and subjected to qualitative and quantitative analysis.
The crude extract of the leaves and stalk of Hippocratea obtusifolia (commonly known as gwadayi in Hausa) was sequentially extracted with petroleum ether, chloroform, ethyl acetate and methanol. The extracts were subjected to bioactivity testing against lactation inducement in 24 female guinea pigs. The experimental animals with average weight of 200g were randomly grouped into six; the first five groups A, B, C, D and E were force-fed with feeds mixed with a measured portion of the extract, while the last group was force-fed with food mixed with distilled water only serving as control. The chloroform extract was found to be the only active extract, and this confirms the use of Hippocratea obtusifolia in lactation inducement.
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