Atopic dermatitis, eczema, allergic dermatitis occupy the main place among dermatoses, where the allergic component is leading in the onset and development of the disease. The most common complication of allergic dermatitis is the attachment of a secondary pyococcus infection, which is associated with a decrease in the antimicrobial resistance of the skin surface. Therapy of infectious lesions is complicated by the increasing resistance of the main pathogens of pyoderma - Staphylococcus aureus and Staphylococcus epidermidis - to widely used antibiotics. The aim of the research: to determine the phenotypic features of staphylococci extracted from patients with allergic dermatitis to assess their pathogenic potential. Materials and methods. The object of the study was 369 staphylococcus isolates removed from affected and intact skin sections of patients with allergic dermatitis, as well as from representative skin sections of healthy individuals undergoing inpatient treatment at the Department of Dermatology of “Institute of Dermatology and Venereology of NAMS of Ukraine”. Biochemical identification and biological properties of staphylococci were determined using methods of classical bacteriology. Results. As a result of the conducted researches, it is established that the complex of phenotypic traits of the removed staphylococcus cultures indicates the presence in the pathogen of factors related to the resistance of the host protection mechanisms and determines the intensity of the alterative action of the infectant in relation to the host organism, the phenotypic manifestation of the studied factors was higher in the staphylococcus isolates removed from the affected skin areas of patients with allergic dermatitis. Conclusions. The level and frequency of phenotypic expression of pathogenicity factors are more pronounced in microorganisms obtained from patients from affected and intact areas compared to controls, which confirms their pathogenetic role in the burden of the disease, which in turn can be used as an auxiliary differential diagnosis criterion.

A serious problem in patients with atopic dermatitis (AD) is the frequent attachment of a secondary skin infection. Among the microbes colonizing the skin of patients suffering from AD, S. aureus takes the lead. According to different authors, from the skin of 80–95 % of patients are sown Staphylococcus aureus. The survival of bacteria in a biotope is promoted by the persistent properties of microorganisms. Aim of the research: to determine the adhesive properties and antilysozyme activity of clinical strains of staphylococci isolated from the skin of patients with allergic dermatosis. The study included 50 patients with atopic dermatitis and 20 practically healthy individuals, from which 140 laboratory strains of staphylococci were isolated: 101 strains from patients with AD and 39 control strains. Bacteriological studies to isolate microorganisms and determine a number of pathogenic characteristics were carried out using the methods of classical bacteriology. The severity of antilysozyme activity (ALA) and adhesive properties of strains isolated from affected areas of the skin was significantly higher than in cultures isolated from intact skin areas, both qualitatively and quantitatively. The obtained data made it possible to assume a certain complicating role of these factors on the course of AD.

Today the one of most important medical-social problems is a growing antibiotic resistance of opportunistic infectious agents. Aim of research: the dynamic observance of antibiotic resistance of opportunistic pathogens – infectious agents of genitourinary tracts under conditions of venereological clinic. The study included 2205 patients with inflammatory diseases of genitourinary tract, who were on the stationary treatment at the department of venereology of SI “IDV NAMS of Ukraine”. Bacteriological studies for isolation of conventionally pathogenic microorganisms with determination of sensitivity of separated laboratory strains to antibacterial drugs of the different groups were carried out using the methods of classic bacteriology. It was demonstrated, that the composition of microbiocenosis of genitourinary tract in patients with infections, caused by conventionally pathogenic microorganisms was characterized by the prevalence of aerobic representatives of Enterobacteriaceae and Staphylococcaceae families that formed associations both between each other and with representatives of other taxonomic groups. The analysis of antibiotic sensitivity of separated strains testifies to the necessity of permanent microbiological monitoring of opportunistic infectious agents for elaborations of the regimes of rational antibacterial therapy.

The expansion of agricultural practices and the raising of animals are key contributors to air pollution. Cattle farms contain hazardous gases, so we developed a cattle farm air pollution analyzer to count the number of cattle and provide comprehensive statistics on different air pollutant concentrations based on severity over various time periods. The modeling was performed in two parts: the first stage focused on object detection using satellite data of farm images to identify and count the number of cattle; the second stage predicted the next hour air pollutant concentration of the seven cattle farm air pollutants considered. The output from the second stage was then visualized based on severity, and analytics were performed on the historical data. The visualization illustrates the relationship between cattle count and air pollutants, an important factor for analyzing the pollutant concentration trend. We proposed the models Detectron2, YOLOv4, RetinaNet, and YOLOv5 for the first stage, and LSTM (single/multi lag), CNN-LSTM, and Bi-LSTM for the second stage. YOLOv5 performed best in stage one with an average precision of 0.916 and recall of 0.912, with the average precision and recall for all models being above 0.87. For stage two, CNN-LSTM performed well with an MAE of 3.511 and an MAPE of 0.016, while a stacked model had an MAE of 5.010 and an MAPE of 0.023.

ДУ «Інститут дерматології та венерології НАМН України»Резюме Мета: вивчення чутливості клінічних ізолятів E. сoli до дії антимікробних препаратів різних класів з визначенням динаміки антибіотикорезистентності за окремі періоди досліджень. Матеріали та методи: проаналізовано результати досліджень рівнів антибіотикорезистентності 241 клінічного ізолята E.сoli, вилучених від хворих на хронічні захворювання сечостатевого тракту. Результати: Дослідження виявили наявність асоційованої резистентності до різних класів антимікробних препаратів у двох третин ізольованих культур ешеріхій. Проведений аналіз чутливості мікроорганізму до антимікробних засобів показав превалювання серед ізолятів антибіотикорезистентних культур з високою питомою вагою полірезистентних штамів. Висновки: Аналіз рівнів антибіотикорезистентності ізольованих клінічних штамів E.сoli продемонстрував високі показники поширення культур з фенотипом MDR та XDR асоційованої резистентності, що виявлена у 63,6% та 7,8% протестованих збудників відповідно. Ключові слова: E.сoli, лабораторні штами, антибіотикорезистентність. Резюме Цель: изучение чувствительности клинических изолятов E. сoli к антимикробным препаратам разных классов с установлением динамики антибиотикорезистентности за отдельные периоды исследований. Материалы и методы: проанализированы результаты исследований антибиотикорезистентности 241 клинического изолята E. сoli, выделенного от больных с хроническими воспалительными процессами мочеполового тракта. Результаты: исследования выявили наличие ассоциированной резистентности к разным классам антимикробных препаратов у двух третей изолированных культур эшерихий. Проведенный анализ чувствительности микроорганизма к антимикробным препаратам показал превалирование среди изолятов антибиотикорезистентных культур с высоким удельным весом полирезистентных штаммов. Ключевые слова: E.сoli, лабораторные штаммы, антибиотикорезистентность. AbstractObjective. Study of susceptibility of the clinical isolates E. coli to antimicrobial drugs of different classes with the antibiotic resistance dynamics determination in some periods of the investigations. Materials and methods. It was studied the antibiotic resistance of 241 strains E. coli isolated from patients with the chronic inflammatory diseases of urogenital tract. Results. The investigations were eliciting the associated resistance to the different classes of the antimicrobial drugs in 2/3 isolated cultures of E.сoli. The analysis of susceptibility to the different antibiotics was demonstrated the antibiotic resistance strains E. coli prevalence with the high level of multidrug resistance. ORCID ID: https://orcid.org/0000-0003-2486-5474Гончаренко Валентина Василівнаканд. мед. наук, наук. співроб. лабораторії мікробіології ДУ «ІДВ НАМНУ» ORCID ID: https://orcid.org/0000-0001-8168-0818 Щоголєва Олена Володимирівнамол. наук. співроб. лабораторії мікробіології ДУ «ІДВ НАМНУ» ORCID ID: https://orcid.org/0000-0002-7235-3556 Іванцова Олена Костянтинівнабактеріолог бак. відділу КДЛ ДУ «ІДВ НАМНУ» ORCID ID: https://...