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Production of antimicrobial agents through the synthesis of gold nanoparticles using green technology has been extensively made consistent by various researchers; yet, this study uses the flower bud's aqueous extracts of Brassica oleracea (Broccoli) as a reducing agent for chloroauric acid (1 mM). After 30 min of incubation, synthesis of gold nanoparticles (AuNps) was observed by a change in extract color from pale yellow to purple color. Synthesis of AuNps was confirmed in UV-visible spectroscopy at the range of approximately 560 nm. The SEM analysis showed the average nanoparticles size of 12-22 nm. The antimicrobial activity of AuNps was analyzed by subjecting it to human pathogenic bacteria (Grampositive Staphylococcus aureus and Gram-negative Klebsiella pneumonia) and fungi (Aspergillus flavus, Aspergillus niger and Candida albicans) using disc diffusion method. The broccoli-synthesized AuNps showed the efficient antibacterial and antifungal activity of above-mentioned microbes. It was confirmed that AuNps have the best antimicrobial agent compared to the standard antibiotics (Gentamicin and Fluconazole). When the concentrations of AuNps were increased (10, 25, and 50 lg/ml), the sensitivity zone also increased for all the tested microbes. The synthesized AuNps are capable of rendering high antimicrobial efficacy and, hence, have a great potential in the preparation of drugs used against major bacterial and fungal diseases in humans.

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In present scenario, ecofriendly approach for the synthesis of noble metals nanoparticles by means of various plant extracts has became a great interest in the field of nanotechnology. This green route schemes are eco-friendly, simple, less toxic and environmental benign. In this paper, an environmental friendly approach is presented for the formation of platinum nanoparticles using aqueous leaf extracts of Indian medicinal herbs such as Jatropa gossypifolia and Jatropa glandulifera as an efficient reducing and capping agents. A great transformation pathway of platinum ions to platinum nanoparticles was obtained by employing the Jatropa gossypifolia and Jatropa glandulifera leaves broth with a reaction temperature of 100 °C. Biosynthesized platinum nanoparticles were successfully characterized by Ultraviolet-Visible spectroscopy. FTIR studies confirmed the presence of amine, amide. The -SO 2 functional group were found to be prone for reduction of platinum metal to platinum nanoparticles . Field Emission Scanning electron microscope (SEM) gives information about the shape and size of the formed nanoparticles. Average platinum nanoparticles particle size distribution ranges from nanometer to micrometer. Transmission electron microscope predominantly indicates the biosynthesized platinum nanoparticles that are dodecahedron and some kind of cubic in nature. In addition, biologically synthesized platinum nanoparticles of leaf extracts exhibits a immense potent of antibacterial activity against pathogenic bacteria's.