Amino acids are the basic structural units of protein molecules in the body. Currently, about 300 amino acids are known, of which 26 are studied best. Amino acids or their derivatives (for example, immune bodies) are part of enzymes, hormones, pigments, and other specific substances that play a crucial role in digestive and metabolic processes. In the process of metabolism, many amino acids are synthesized in the body from other amino acids or compounds, and therefore they are called interchangeable. Amino acids that are not synthesized in the body or are formed in insufficient quantities are called indispensable. According to the content and ratio of essential amino acids, feed proteins are subdivided into full and inferior. Deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. The following amino acids are indispensable; arginine, viburnum, histidine, isoleucine, leucine, lysine, methionine, threonine, tryptophan, phenylalanine. Among the essential amino acids, especially important ones, are those called critical. These are lysine, methionine + cystia, threonine, and tryptophan. A deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. Research and production experiment was conducted within Olenka Poultry Factory LLC and the problematic research laboratory of feed additives of the Department of Animal Feeding and Feed Technology named after P. D. Pshenichny at the National University of Life and Environmental Sciences. The effective level of arginine in compound feed for broilers of the Cobb-500 cross was experimentally determined. The use of compound feed with arginine levels in the first rearing period (1 to 10 days) - 1.28%, in the second (11 to 22 days) - 1.15% and in the third (23 to 42 days) - 1.11% gives the opportunity to get broiler chickens at the age of 42 days, weighing 2.654 kg, at a feed expenditure of 1.78 kg per 1 kg of gain.
Within the framework of these studies during 2014-2015, the levels of extensiveness and intensity of infection by various types of helminths were studied for three age groups of chickens from local breeds, reared in outdoor stabling conditions and walking areas of family poultry farms in mountainous (Khizi district), foothill (Khachmaz district) and lowland (Saatly district) territories. In studies carried out in these three climatic zones, intense infection of birds with helminths at 3-4 months of age, making uo withA.galli EI 62.3, H. gallinarum EI 50.8, II 1-30, R.tetragona IE 36, 6, 1-14, S.trachea EI 29.1, C.obsignata EI 17.7 %, II 1-13, in 5-7 months of age Sinqamus II 1-19, in adults Askaridia II 1-36, were recorded in foothill areas. A low level of extensiveness and intensity of invasion of infected birds was found in lowland areas, and a medium level in foothill areas. One of the main reasons for this is low temperature and high relative humidity in mountainous areas, high air temperature and low relative humidity in low-lying areas and a suitable air temperature and relative humidity in foothill areas for the development of helminth eggs in the external environment.
The aim of the research is to study the effect of “Therapeutic and preventive immunoglobulin” immunostimulant on the veterinary and sanitary assessment of meat and the histological features of the parenchymatous organs of cattle. The study showed that pH of the meat in the experimental group was 5.32±0.03, in the control group – 5.43±0.02, the level of amine ammonia nitrogen – 1.19±0.02 and 1.21±0.01 mg in the experimental and control groups, respectively. The peroxidase reaction was positive both in the experimental and control groups, the reaction with copper sulfate was negative. The copper content was 34% more in the experimental group than in the control group (1.80±0.03 mg/kg). The concentration of zinc was 38.5±1.32 and 23.1±0.98 mg/kg in the experimental and control groups, respectively. The levels of iron were similar in the experimental and control groups. The cobalt content in the meat of calves in the experimental group was 24.6% higher than in the control group. The concentration of selenium and magnesium in the meat of calves in the experimental group was higher than in the control group. Such heavy metals as lead, cadmium, mercury and arsenic were not found in the meat of the calves in the experimental and control groups. According to the results of the histological study, the drug does not cause pathological changes in the structure of the liver, kidneys, myocardium, lymph nodes and spleen.
The article deals with the autopsy materials of the Friesian breed domestic horse (Equus ferus caballus). It presents the research on tissues’ and organs’ histological examination as well as materials from the case record. New nosology for this species and breed has been revealed for the first time for the animal under examination. It was chronic inflammatory thyroid disease of the autoimmune genesis in the hyperthyroid stage which caused the systemic idiopathic fibrosis. Previously this nosology was neither revealed nor described in the Russian and foreign literature sources. The article presents materials from the case record where animal’s clinical condition has been assessed. The description of pathoanatomical and morphological changes in the thyroid tissues in the detected autoimmune thyroiditis and associated pathological processes in the organs and tissues of the studied animal was given. The systemic idiopathic fibrosis associated with thyroiditis condition was described. The features of the blood serum metabolic profile according to the enzymes content, substrates and minerals were presented. The assessment of the thyroid gland endocrine status of total thyroxine (tT4) and total triiodothyronine (tT3) has been made. The level of which was 184.0 nmol/l and 7.9 nmol/l respectively. The data indicating the presence of an autoimmune process in relation to thyroid antigens have been traced. The level of antibodies to thyroglobulin and antibodies to thyroperoxidase was 9.7 IU/ml and 98 IU/ml respectively.
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