Objective: Methodology development for quality control of optically active pharmaceutical substances based on water isotopologues.Methods: Solutions of L-ascorbic acid, glucose, galactose and valine stereoisomers were prepared using deuterium depleted water (DDW-«light» water, D/H=4 ppm), natural deionized high-ohmic water (BD, D/H=140 ppm), heavy water (99.9% D2O). The optical rotation was observed using an automatic polarimeter Atago POL-1/2. The size distribution of giant heterogeneous clusters (GHC) of water was recorded by low angle laser light scattering (LALLS) method.Results: The infringement of Biot’s Law was found for solutions of ascorbic acid, expressed in the absence of a constant value of the specific optical rotation  at a concentration of below 0.1%, depends on the D/H ratio. The inequality was established in absolute values of optical rotation for L-and D-isomers of valine in solutions with different ratios of hydrogen isotopologues. The mutarotation of glucose confirmed the first-order kinetics, and the activation energies were statistically distinguishable for BD and DDW. The mutarotation of the natural galactose D-isomer proceeded with a lower energy consumption compared to the L-isomer. In heavy water, the mutarotation of monosaccharides had different kinetic mechanisms. Polarimetric results correlated with the number and size of GHC, which confirmed the possibility of chiral solvent structures induction by optically active pharmaceutical substances.Conclusion: In the optically active pharmaceutical substances quality control there should be considered the contribution of induced chiral GHC of water to the optical rotation value that depends on the isotopic D/H ratio, the substance nature and the form of its existence at a given pH.

There are many references in the literature related to connection between the space weather and the state of human organism. The search of external factors influence on humans is a multi-factor problem and it is well known that humans have a meteo-sensitivity. A direct problem of finding the earth weather conditions, under which the space weather manifests itself most strongly, is discussed in the present work for the first time in the helio-biology. From a formal point of view, this problem requires identification of subset (magnetobiotropic region) in three-dimensional earth's weather parameters such as pressure, temperature, and humidity, corresponding to the days when the human body is the most sensitive to changes in the geomagnetic field variations and when it reacts by statistically significant increase (or decrease) of a particular physiological parameter. This formulation defines the optimization of the problem, and the solution of the latter is not possible without the involvement of powerful metaheuristic methods of searching. Using the algorithm of differential evolution, we prove the existence of magnetobiotropic regions in the earth's weather parameters, which exhibit magneto-sensitivity of systolic, diastolic blood pressure, and heart rate of healthy young subjects for three weather areas (combinations of atmospheric temperature, pressure, and humidity). The maximum value of the correlation confidence for the measurements attributable to the days of the weather conditions that fall into each of three magnetobiotropic areas is an order of 0.006, that is almost 10 times less than the confidence, equal to 0.05, accepted in many helio-biological researches.

In two series of experiments on cats, afferent reactions of organs of the gastroduodenal complex (gastric corpus, pylorus, and duodenal bulb) were studied and myoelectric activity of these portions of the gastroduodenal complex was examined. In each series, only one portion of the gastroduodenal complex was stimulated with electrical pulses. It was found that processes underlying changes in functional heterogeneity and enhancement or moderation of their interrelations in the acute damage period can affect the development of early regional adaptation.

Acute experiments on cats demonstrated facilitation effects of afferent reactions, and segmentary motor reactions during stimulation of sciatic nerves and inhibition of corticofugal motor reactions induced by electrical stimulation of ilium osteoreceptors. Key Words: nociception; intraosseous receptors; radicular syndromeThe osteoreceptor system remains one of the least studied parts of sensory system physiology. This might be due to the fact that so far no holistic concept has yet emerged regarding its role in physiological functions and its participation in the pathogenesis of somatic and visceral diseases [3,4]. Published data suggest that overstimulation of intraosseous receptors (e.g., during increased intraosseous pressure) may cause disturbances in sensorirnotor reactions, most clearly manifested in the general segments of spinal innervation [1][2][3][4]. Increased intraosseous afferentation is thought to play an essential role in the development of neurologic manifestations of lumbar osteochondrosis, prompting the present study aimed at elucidating the effect of osteoreceptor system stimulation on the formation of evoked potentials (EP) in the cerebral cortex and electromyographic reactions for discrete electrostimulations of the sciatic nerve. MATERIALS AND METHODSEP in the focus of maximum activity of the first sensorimotor area of the cerebral cortex and the electromyogram (EMG) of femora/ muscles during sciatic nerve stimulation (pulse duration 0. cats (14 animals) under chloralose anesthesia and myorelaxation. Electrostimulation was applied to motor zones of the cortex and the EMG-equivalent of the femoral muscle motor reaction was recorded. Conditioning stimulations were applied to the spongy substance of the ilium by means of two needle electrodes insulated their entire length except for the ends, introduced into the spongy bone at a depth of 3-4 mm and 3-4 mm apart (pulse duration 0.3 msec, strength 3-10 xmA). Experiments were performed using stereotaxis with Multibasis multipurpose neurophysiological apparatus (Biomedica) for 10 signal presentations. The amplitude fluctuations of the summated phases (from peak to peak) were subjected to statistical processing: 1-2 EP phases and deviations from the EMG response.The strength of sciatic nerve stimulation was selected at the level of threshold intensity sufficient for forming stable responses. For the sake of convenience the alterations of the indexes obtained for conditioning stimulations of the spongy substance of the ilium were estimated in percentages, taking the initial data as 100%. The interval between conditioning and test stimuli ranged from 100 to 1000 msec. RESULTSConditioning stimulations of the spongy substance of the ilium markedly affected the amplitude of the test EP and of the EMG response. For example, within the interval from 300 to 700 msec the summated

Cerebral hyperthermia is a factor of pathogenesis of secondary brain injury. Microwave recording of temperature allows to identify thermal anomalies in the brain while craniocerebral hypothermia arrests their development. Craniocerebral hypothermia has marked neuroprotective effects in patients with brain lesions.