A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria

Lee, Seonghwan; Song, Kyung–Mi; Jeon, Weejeong; Jo, Hang-Hyun; Shim, Yoon‐Bo; Ban, Changill

doi:10.1016/j.bios.2012.03.003

Cited by 94 publications

(88 citation statements)

References 24 publications

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“…Their aptamers were distinct from those presented here, yet sequence homology shows they may share some structural similarities with a likely stem loop, although they did not investigate the structure of their aptamers (27). Dissociation constants for the interaction with PfLDH were in a similar range (16.8-49.6 nM) to those we observe here.…”

Section: Discussionsupporting

confidence: 63%

“…Dissociation constants for the interaction with PfLDH were in a similar range (16.8-49.6 nM) to those we observe here. Using an aptasensor linked to an electrochemical impedance spectroscopy assay they could demonstrate a detection limit of 1 pM (27), and detected both P. vivax and Plasmodium falciparum at less than 100 parasites per microliter. The combination of their results, together with the structural insight we provide herein, provides a strong argument that aptamer-mediated molecular recognition could have potential for the diagnosis of malaria.…”

Section: Discussionmentioning

confidence: 99%

“…A group recently published a pair of papers identifying aptamers against PfLDH (27) and against Plasmodium vivax LDH (28). Their aptamers were distinct from those presented here, yet sequence homology shows they may share some structural similarities with a likely stem loop, although they did not investigate the structure of their aptamers (27).…”

Section: Discussionmentioning

confidence: 99%

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Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer

Cheung¹,

Kwok²,

Law³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

117

122

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Significance Aptamers are oligonucleotides selected and evolved to bind tightly and specifically to molecular targets. Aptamers have promise as diagnostic tools and therapeutic agents, but little is known about how they recognize or discriminate their targets. In this study, X-ray crystallography together with several other biophysical techniques reveal how a new DNA aptamer recognizes and discriminates Plasmodium lactate dehydrogenase, a protein marker that is a diagnostic indicator of infection with the malaria parasite. We also demonstrate application of the aptamer in target detection. This study broadens our understanding of aptamer-mediated molecular recognition and provides a DNA aptamer that could underpin new innovative approaches for point-of-care malaria diagnosis.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer

Cheung¹,

Kwok²,

Law³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

117

122

View full text Add to dashboard Cite

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“…Single-stranded random DNA library with the design 5 0 -CACCTAATACGACTCACTATA GCGGATCCGA-N40-CTGGCTCGAACAAGCTTGC-3 0 was obtained from IDT [16]. Before use, the library was denatured at 90°C for 3 min, followed by incubation at 4°C for 5 min.…”

Section: In Vitro Selection Of Dna Aptamersmentioning

confidence: 99%

“…Several biomarkers for diagnosis of malaria have been investigated [13] among which Plasmodium-specific lactate dehydrogenase has emerged as a promising biomarker for malaria detection owing to its subtle structural and kinetic differences from the mammalian counterpart, leading to an increased interest in development of sensors that target this enzyme [14][15][16][17][18]. Here, we report a novel ssDNA aptamer developed against Plasmodium falciparum lactate dehydrogenase and its application potential to detect this biomarker through an aromatic cationic surfactant-based assay using gold nanoparticles as the optical sensing platform.…”

Section: Introductionmentioning

confidence: 99%

Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

et al. 2016

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A novel ssDNA aptamer (P38), with a 40 mer random region flanked by primer-binding sites on both sides, targeting Plasmodium falciparum lactate dehydrogenase (PfLDH) has been developed through systematic evolution of ligands by exponential enrichment (SELEX), including counter SELEX against human lactate dehydrogenase A and B (hLDH A and B). The 2D structure of P38 shows the presence of three stem loops with a δG of -9.18 kcal/mol. EMSA studies on P38 complexes with the increasing concentration of PfLDH revealed a dissociation constant of 0.35 µM. P38 has been exploited for the quantitative detection of PfLDH using cationic surfactant-mediated aggregation of gold nanoparticles (16-nm diameter) as an optical probe. Among the three different cationic surfactants, characterized by different hydrocarbon tail groups, benzalkonium chloride (BCK) was found to be most efficient with a limit of detection of 281 ± 11 pM. This BCK-based approach with the novel highly selective aptamer provides simple and sensitive detection of PfLDH in the clinically relevant range.

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2D Ternary Chalcogenides

Gao

Zhang

et al. 2018

Advanced Optical Materials

133

100

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Crystal structure and composition are strongly correlated to materials' properties and functionalities. In this section, we first introduce the crystal structures and corresponding properties 2D ternary chalcogenides have been extensively studied by researchers from materials science and nanotechnology because of their unique structures, novel chemical and physical properties, and great potential in nanoscale device applications. With the addition of a third element, these 2D ternary chalcogenides are anticipated to be more appealing because of the stoichiometric variation and synergistic effect compared to their binary counterparts. The novel properties of 2D ternary chalcogenides have endowed various applications in electronics, optoelectronics, biosensors, and catalysis. Here, a summary is given of the latest research progress of 2D ternary chalcogenides from the aspects of preparation, characterization, and application. The article is concluded with a screening of the existing challenges and an outlook for these rising 2D ternary materials.

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A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria

Cited by 94 publications

References 24 publications

Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer

Structural basis for discriminatory recognition of Plasmodium lactate dehydrogenase by a DNA aptamer

Aromatic Surfactant as Aggregating Agent for Aptamer-Gold Nanoparticle-Based Detection of Plasmodium Lactate Dehydrogenase

2D Ternary Chalcogenides

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