Application of Response Surface Methodology to Improve Carotene Production from Synthetic Medium by Blakeslea trispora in Submerged Fermentation

Filotheou, Andreas; Nanou, Konstadina; Papaioannou, Emmanouil H.; Roukas, T.; Kotzekidou, P.; Liakopoulou-Kyriakides, M.

doi:10.1007/s11947-010-0405-6

Cited by 17 publications

(7 citation statements)

References 27 publications

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“…Only Fe 2+ and Fe 3+ improved the pigment production, while other metal ions inhibited the production of β-carotene. The positive influence of Fe 3+ on carotene production was found by Filotheou et al (2012). It might be attributed to the high amount of hydroxyl radical generated by the Fenton reaction, since the stimulation of biosynthesis of carotenes by oxidative stress has been observed in Blakeslea trispora (Nanou and Roukas, 2011).…”

Section: Optimization Of β-Carotene Productionmentioning

confidence: 91%

Poly (l-lactide acid) improves complete nano-hydroxyapatite bone scaffolds through the microstructure rearrangement

Wang

Lin

et al. 2012

Electron. J. Biotechnol.

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β-carotene is a commonly used food colorant. In this work, a novel β-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of β-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for β-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe 2+ , pH 6.0 and 30ºC. Under the optimal conditions, the yield of β-carotene achieved 2.45 µg/mL.

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Section: Optimization Of β-Carotene Productionmentioning

confidence: 91%

Poly (l-lactide acid) improves complete nano-hydroxyapatite bone scaffolds through the microstructure rearrangement

Wang

Lin

et al. 2012

Electron. J. Biotechnol.

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“…Response surface methodology (RSM), a statistical technique used to investigate and optimize several complex processes (Filotheou et al 2012), was employed in the present study. Among the RSM designs, the Box-Behnken design (BBD) requires the fewest runs in experimental design (Ay et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Rapid and environmentally-friendly extraction of carotenoids from Blakeslea trispora

et al. 2015

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“…The selection of suitable carbon sources could have a considerable effect on the overall productivity, peak titer of secondary metabolites, and cost of raw material [ 27 ]. During microbial fermentation, a carbon source acts as a significant component for the construction of cellular material and an energy provider as well for microbial growth [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Naz

Nazir

Nosheen

et al. 2020

BioMed Research International

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Carotenoids produced by microbial sources are of industrial and medicinal importance due to their antioxidant and anticancer properties. In the current study, optimization of β-carotene production in M. circinelloides strain 277.49 was achieved using response surface methodology (RSM). Cerulenin and ketoconazole were used to inhibit fatty acids and the sterol biosynthesis pathway, respectively, in order to enhance β-carotene production by diverting metabolic pool towards the mevalonate pathway. All three variables used in screening experiments were found to be significant for the production of β-carotene. The synergistic effect of the C/N ratio, cerulenin, and ketoconazole was further evaluated and optimized for superior β-carotene production using central composite design of RSM. Our results found that the synergistic combination of C/N ratios, cerulenin, and ketoconazole at different concentrations affected the β-carotene productions significantly. The optimal production medium (std. order 11) composed of C/N 25, 10 μg/mL cerulenin, and 150 mg/L ketoconazole, producing maximum β-carotene of 4.26 mg/L (0.43 mg/g) which was 157% greater in comparison to unoptimized medium (1.68 mg/L, 0.17 mg/g). So, it was concluded that metabolic flux had been successfully redirected towards the mevalonate pathway for enhanced β-carotene production in CBS 277.49.

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Application of Response Surface Methodology to Improve Carotene Production from Synthetic Medium by Blakeslea trispora in Submerged Fermentation

Cited by 17 publications

References 27 publications

Poly (l-lactide acid) improves complete nano-hydroxyapatite bone scaffolds through the microstructure rearrangement

Poly (l-lactide acid) improves complete nano-hydroxyapatite bone scaffolds through the microstructure rearrangement

Rapid and environmentally-friendly extraction of carotenoids from Blakeslea trispora

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

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