Bone apposition to laminin-1 coated implants: histologic and 3D evaluation

Bougas, Kostas; Jimbo, Ryo; Vandeweghe, Stefan; Hayashi, Mariko; Bryington, Matthew; Kozai, Yusuke; Schwartz-Filho, Humberto Osvaldo; Tovar, Nick; Adolfsson, Erik; Ono, Daisuke; Coelho, Paulo G.; Wennerberg, Ann

doi:10.1016/j.ijom.2012.11.008

Cited by 14 publications

(8 citation statements)

References 33 publications

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“…Commonly utilized modifications that enhance the biological response to implants are alterations in surface texture and chemistry . Moreover, recent studies indicate that the addition of bioactive substances may increase the osteoconductive property if the implant …”

Section: Introductionmentioning

confidence: 99%

The in vivo effect of P‐15 coating on early osseointegration

et al. 2013

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The aim of this study was to evaluate mechanically and morphologically the effect of a specific peptide sequence P-15, when incorporated into implant surfaces. Three types of implants were used for the study: Group A: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition, Group B: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition + P-15 incorporation, and as control, Group C: commercially pure titanium implant (blasted and acid etched). After a topographical characterization, transcortical osteotomies were made, and all implant groups (102 implants per group) were randomly placed bilaterally in the tibiae of adult beagle dogs (n = 24). At, 1, 2, and 4 weeks post-surgery, the animals were sacrificed and the samples were retrieved for removal torque tests, for nano indentation, and for histomorphometrical analysis. The results (mean ± 95% CI) showed that Group B (34.4 ± 8.7%) presented statistically higher bone-to-implant contact than the other groups (A = 23.9 ± 7.8%; C = 21.7 ± 8.3%) at 1 week, indicating an enhanced osteogenesis due to the peptide incorporation. The results suggested that the incorporation of P-15 to implant surfaces increased its bioactivity and the effects were notable especially in the early stages of the healing process.

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Section: Introductionmentioning

confidence: 99%

The in vivo effect of P‐15 coating on early osseointegration

et al. 2013

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“…While it is known to be involved in adhesion across a broad range of cell types [2], Ln2-P3 has been chiefly evaluated in terms of its effectiveness in promoting epithelial cell attachment rather than in estimating its affinity for bone cells [10–12]. A few studies have been found to test the bone response of a laminin-coated titanium (Ti) surface [13–16]. However, the coated material has been laminin, the protein itself, not a peptide derived from it.…”

Section: Introductionmentioning

confidence: 99%

Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

Min

Kang

Jang

et al. 2013

BioMed Research International

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Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.

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“…However, these studies primarily used smooth Ti surface implants for in vitro and in vivo experiments. Only two experiments assessed the effect of a laminin coating on modified Ti surfaces, and these modifications comprised a hydroxyapatite coating and alkali and heat treatments (Bougas et al, , ). In contrast, we assessed the effect of treating SLA surfaces with a human laminin α2 chain‐derived peptide on the osseointegration of implants.…”

Section: Discussionmentioning

confidence: 99%

A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

Choi

Kim

et al. 2020

J Biomedical Materials Res

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Early implant loading is very important for reducing the duration of missing teeth in human patients. The laminin‐derived peptide, DLTIDDSYWYRI motif (Ln2‐P3), accelerates bone healing. Therefore, to investigate the hypothesis that Ln2‐P3 increases the bone response to sandblasted, large‐grit, acid‐etched (SLA) titanium implants, the effect of the Ln2‐P3 peptide on the osseointegration of SLA titanium implants was evaluated in vitro and in vivo. Human osteoblast‐like cells were cultured on untreated, scrambled peptide (SP)‐treated, and Ln2‐P3‐treated SLA titanium discs, and the cellular responses of these cells were evaluated. The Ln2‐P3 treatment augmented osteoblast attachment and spreading, alkaline phosphatase activity, and the expression of osteogenic marker genes. Furthermore, the untreated and Ln2‐P3‐treated SLA titanium implants were inserted into the tibiae of rabbits for 9 and 11 days. Compared with the untreated implants, the Ln2‐P3‐treated implants showed a significantly higher bone‐to‐implant contact ratio at Day 9 after implantation and an increased bone area. The Ln2‐P3 treatment of the SLA titanium implant surface augmented osteoblastic activity and accelerated peri‐implant bone formation at the bone–implant interface. Overall, these results indicated that compared with the SLA titanium surface alone, the Ln2‐P3 peptide‐treated SLA titanium surface enhances initial osseointegration, thereby facilitating earlier implant loading.

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Bone apposition to laminin-1 coated implants: histologic and 3D evaluation

Cited by 14 publications

References 33 publications

The in vivo effect of P‐15 coating on early osseointegration

The in vivo effect of P‐15 coating on early osseointegration

Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

A laminin‐211‐derived bioactive peptide promotes the osseointegration of a sandblasted, large‐grit, acid‐etched titanium implant

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