Cytotoxic Activity of Ethanol and Ethyl Acetate Extract of Kenikir (Cosmos caudatus) against Cervical Cancer Cell Line (HELA)

Cosmos caudatus (Asteraceae), is known as Ulam raja in Malaysia or kenikir in Indonesia also sometimes referred to as "King's salad." It is usually consumed as a salad and the leaves have been widely used as a traditional medicine due to their pharmacological activities and beneficial effects on human health. The leaves have been reported to contain several phytoconstituents such as flavonoids and their derivatives, other phenolics, and essential oil, while the roots only contain non-flavonoids. Furthermore, the leaves have been reported to contain a high total phenolic content (TPC) which is attributable for various activities including antidiabetes, antioxidant, anti-inflammation, antibacterial, antifungal, antiosteoporosis, anti-hyperlipidemic, anticancer, antihypertensive, anti-hepatoprotective, and to manage fertility problems. However, further research needs to be done in order to determine whether C. caudatus is effective in treating thrombolytic and leishmanial disorder. Clinical study regarding the use of C. caudatus as an antidiabetic agent has been reported as a supplement to improve insulin resistance and sensitivity in type 2 diabetes patients [NCT02322268]. The findings of the toxicity tests revealed that the leaves are nontoxic and that they can be taken without risk. It is still need to conduct an additional in vitro and in vivo investigations to confirm various traditional claims about the therapeutic potential of this plant in the treatment of various ailments. This traditional medicinal plant's genuine medicinal benefit will be further confirmed by additional clinical trials and toxicity assessments.

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A review on Cosmos caudatus as A potential medicinal plant based on pharmacognosy, phytochemistry, and pharmacological activities

Ahda

Jaswir

Khatib

et al. 2023

International Journal of Food Properties

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Effect of Zanthoxylum acanthopodium methanol extract on CDK4 expression to cervical cancer

Simanullang¹,

Hutahaean

Rosidah

et al. 2021

RJPT

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Cervical cancer is a disease from the Human papillomavirus (HPV) through transmission, virus persistence, clone development until infecting the cells in the cervical. This study is to determine CDK4 expression in cervical cancer cells after being given Zanthoxylum acanthopodium methanol extract (ZAM) and the histological description of cervical cancer cells. This study consisted of 5 treatment groups. K-: control group, K+: rats model of cancer, P1: rats model of cancer with a dose of 100mg/BW of ZAM, P2: rats model of cancer with a dose of 200 mg/BW of ZAM, and P3: rats model of cancer with a dose of 400 mg/BW of ZAM. The cervical tissue was prepared on paraffin blocks and given Immunohistochemistry staining. Results showed that the expression of CDK4 are reduced in the ZAM treatment at doses of 200 and 400mg/KgBW (P<0.05) in cervical histology, but in doses of 100mg/kg BW, many brown marks are still visible on the cervical tissue. These proteins will bind, inhibit proteins, cell cycle development, modulate cell division, and signal transduction pathways of apoptotic signaling. The injection of benzopyrene and given ZAM in cervical tissue affect hematological values. ZAM affects and improves cervical histology after benzopyrene injection. The extract of andaliman can be developed into a cervical cancer drug candidate.

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In vitro and in silico Anti-dengue activity of Supercritical extract of medicinal plants against Dengue serotype-2

Dar

Kaushik

Kumar

et al. 2021

RJPT

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Dengue is transmitted by female Aedes mosquitoes. It has been reported that about 2.5 billion peoples are at the risk of dengue virus. Millions of cases of dengue virus occur worldwide each year. There is no antiviral drug available still. Hence, the researchers are in the search of new anti-dengue drugs from natural products. The present study is aimed to determine the anti-dengue activity of supercritical extracts of Andrographis paniculata, Berberis vulgaris, Carica papaya, Euphorbia hirta, Phyllanthus niruri and Tinospora cordifolia in vitro and in silico. The cell viability was evaluated in C6/36 cells line by using MTT assay using a microplate reader at 595 nm. The maximum non-toxic dose of C. papaya and B. vulgaris extracts were reported as 46.87µg/ml, 31.25µg/ml for A. paniculata, P. niruri and E. hirta and 23.43 µg/ml, for T. cordifolia. Further, the anti-dengue activity of plants extract was analyzed by real-time RT-PCR. 100 copies/ml of DENV-2 virus was used for the antiviral assay. A. paniculata supercritical extract showed complete inhibition against the dengue-2 virus at 40ºC temperature and 15Mpa pressure. The other plant extracts showed the inhibition in order of T. cordifolia (83.44%) > C. papaya (34.71%) > E. hirta (28.70%) whereas P. niruri and B. vulgaris failed to inhibit dengue virus. Andrographolide a major compound present in A. paniculata has been reported to have antiviral activity against hepatitis B, C virus, herpes simplex virus, influenza virus, chikungunya virus, dengue virus 2 and 4. Results of molecular docking showed that the interaction between andrographolide and NS1protein shows the maximum binding energy -7.30 Kcal/mol. The docking study was conducted to validate the result against the anti-dengue activity using dengue NS1 protein with andrographolide. It was concluded that A. paniculata could be a source for isolation of therapeutic compounds against the dengue-2 virus.

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Cytotoxic Activity of Ethanol and Ethyl Acetate Extract of Kenikir (Cosmos caudatus) against Cervical Cancer Cell Line (HELA)

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A review on Cosmos caudatus as A potential medicinal plant based on pharmacognosy, phytochemistry, and pharmacological activities

A review on Cosmos caudatus as A potential medicinal plant based on pharmacognosy, phytochemistry, and pharmacological activities

Effect of Zanthoxylum acanthopodium methanol extract on CDK4 expression to cervical cancer

In vitro and in silico Anti-dengue activity of Supercritical extract of medicinal plants against Dengue serotype-2

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