Effect of dielectric barrier discharge plasma jet on bactericidal and human dermal fibroblasts adult cells: In vitro contaminated wound healing model

Poramapijitwat, Pipath; Thana, Phuthidhorn; Boonyawan, Dheerawan; Janpong, Keratiya; Kuensaen, Chakkrapong; Charerntantanakul, Wasin; Yu, L.D.; Sarapirom, S.

doi:10.1016/j.surfcoat.2020.126482

Cited by 6 publications

(1 citation statement)

References 35 publications

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“…Similar to a different study, regular DBD therapy at both frequencies revealed disturbed biofilms and cells that seemed to have structural damage [ 38 ], and the reason for this may be the use of gas in the plasma, which increases the production of chemically reactive species, thereby increasing the antimicrobial effect. Previously, Lee et al [ 39 ] reported that FE-SEM analysis of atmospheric pressure plasma jet-treated biofilms of Gram-positive and Gram-negative bacteria on a titanium surface revealed decomposed cell membranes during treatment and severe damage after treatment [ 40 ]. Several studies have demonstrated the effectiveness of biofilm reduction using UV-C, a sterilization technique similar to DBD plasma [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dielectric Barrier Discharge Plasma against Listeria monocytogenes Mixed-Culture Biofilms on Food-Contact Surfaces

et al. 2023

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Listeria monocytogenes is a major foodborne pathogen. Various methods can be used to control biofilms formed by foodborne pathogens. Recently, the food industry has become interested in plasma, which can be used as a non-thermal technology with minimum changes to product quality. In this study, the effects of dielectric barrier discharge (DBD) plasma on L. monocytogenes mixed-culture biofilms formed on stainless steel (SS), latex hand glove (HG), and silicone rubber (SR) were investigated. DBD plasma effectuated reductions of 0.11–1.14, 0.28–1.27 and 0.37–1.55 log CFU/cm2, respectively. Field emission scanning electron microscopy (FE-SEM) demonstrated that DBD plasma cuts off intercellular contact and induces cell decomposition to prevent the development of biological membranes. It was confirmed that the formed biofilms collapsed and separated into individual bacteria. Our findings suggest that DBD plasma can be used as an alternative non-heating sterilization technology in the food industry to reduce biofilm formation on bacterial targets.

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