Effects of Omeprazole and Caffeine Alone and in Combination with Gentamicin and Ciprofloxacin Against Antibiotic Resistant <i>Staphylococcus Aureus</i> and Escherichia Coli Strains

Bazzaz, Bibi Sedigheh Fazly; Fakori, Mahmoud; Khameneh, Bahman; Hosseinzadeh, Hossein

doi:10.3831/kpi.2019.22.006

Cited by 30 publications

(18 citation statements)

References 21 publications

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“…The methanolic extract of cocoa pod husk has shown inhibitory and bactericidal activity against Staphylococcus aureus at a concentration of 0.62 mg/mL and 5 mg/mL, respectively [ 65 ]. In addition, pure caffeine has been shown to exert inhibitory effects against Staphylococcus aureus at a concentration of 200 μg/mL [ 77 ]. These differences might be attributed to the relatively low concentrations of both cocoa extract (0.2% w / v ) and caffeine anhydrous (0.05% w / v ) in the beverage formulation.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

Kolonas

Vareltzis

Kiroglou

et al. 2023

IJMS

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Athletes often consume functional beverages in order to improve performance and reduce oxidative stress caused by high-intensity exercise. The present study aimed to evaluate the antioxidant and antibacterial properties of a functional sports beverage formulation. The beverage’s antioxidant effects were assessed on human mesenchymal stem cells (MSCs) by determining thiobarbituric acid reactive substances (TBARS; TBARS levels decreased significantly by 52.67% at 2.0 mg/mL), total antioxidant capacity (TAC; TAC levels increased significantly by 80.82% at 2.0 mg/mL) and reduced glutathione (GSH; GSH levels increased significantly by 24.13% at 2.0 mg/mL) levels. Furthermore, the beverage underwent simulated digestion following the INFOGEST protocol to assess its oxidative stability. The analysis of the total phenolic content (TPC) using the Folin–Ciocalteu assay revealed that the beverage contained a TPC of 7.58 ± 0.066 mg GAE/mL, while the phenolics identified by HPLC were catechin (2.149 mg/mL), epicatechin (0.024 mg/mL), protocatechuic acid (0.012 mg/mL), luteolin 7-glucoside (0.001 mg/mL), and kaempferol-3-O-β-rutinoside (0.001 mg/mL). The beverage’s TPC was strongly correlated with TAC (R2 = 896). Moreover, the beverage showcased inhibitory and bacteriostatic effects against Staphylococcus aureus and Pseudomonas aeruginosa. Lastly, the sensory acceptance test demonstrated that the functional sports beverage was well accepted by the assessors.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

Kolonas

Vareltzis

Kiroglou

et al. 2023

IJMS

View full text Add to dashboard Cite

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“…The results show that 45% gentamicin sulfate inhibited the growth of E. coli , S. aureus , and B. cereus . Badenoch et al showed that gentamicin sulfate is effective in killing P. aeruginosa with an MBC value of 4 μg/mL [ 12 ], while Bazzaz et al reported MBC values of 80 μg/mL and 0.625 μg/mL for gentamicin sulfate against S. aureus and P. aeruginosa , respectively [ 11 ]. Riwom et al reported an MBC value of 30 μg/mL gentamicin sulfate against B. cereus , S. typhi , S. aureus , Shigella spp, and E. coli [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

“…Drug formulations in the form of nanosprays based on self nanoemulsifiying drug delivery systems (SNEDDS) have been prepared based on the optimal dose of the leaf fraction of M. malabathricum with antibiotics, gentamicin and ciprofloxacin. Such formulations aim to reduce the severity of the continued infection of DFU wounds and can be effective in the treatment of resistant bacteria [ 11 ]. These nanospray preparations are based on the dosages of antibiotics and their combinations with the extract determined based on the value of the Fractional Inhibitory Concentration Index (FICI) used to define additivity or synergism [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Highest Dosage Combination Activity Screening from the Leaf Fraction of Melastoma malabathricum with Antibiotic Gentamicin and Ciprofloxacin

2022

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Objectives: This study aims to determine the Fractional Inhibitory Concentration Index (FICI) of combinations of Melastoma malabathricum leaf fraction with ciprofloxacin or gentamicin against pathogenic bacteria, Escherichia coli, Staphylococcus aureus, and Bacillus cereus, isolated from Diabetic Foot Ulcer (DFU) patients. Methods: We tested concentrations of 45%, 55%, 65%, and 75% of gentamicin and ciprofloxacin using dilution and agar diffusion methods. The combination of M. malabathricum leaf extract with these antibiotics was tested in vitro against all three bacteria. Results: The combination of M. malabathricum leaf extract and ciprofloxacin gave a FICI value of 0.5, indicating synergistic antibacterial activity against the test bacteria. Conclusion:The results show that the antibacterial effect of a combination of high doses of the leaf extract with either antibiotic is greater than that of the leaf extract and the antibiotics in single use.

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“…Multiple mechanistic pathways have been suggested to explain the antimicrobial effects of caffeine. For instance, caffeine might inhibit microbial DNA synthesis by suppressing thymidine kinase and preventing the inclusion of thymidine or adenine bases, which might additionally potentiate the effects of other antimicrobial agents [ 37 , 38 ]. Furthermore, the antimicrobial effects of caffeine might be indirectly augmented by its combination with other antimicrobial agents that target microbial cell-wall synthesis, which facilitates the influx of caffeine into microorganisms [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Antifungal and Antibiofilm Activities of Caffeine against Candida albicans on Polymethyl Methacrylate Denture Base Material

et al. 2022

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Background: In this study, the effect of pure caffeine was established against Candida albicans (C. albicans) using different microbiological techniques. Methods: Broth microdilution and colony forming units (CFUs) assays were used to detect the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The Live/Dead fluorescent dyes were implemented to determine the yeast viability. Polymethyl methacrylate acrylic resin (PMMA) discs were prepared to evaluate caffeine’s effects against adherent C. albicans using microplate reader, CFUs, and scanning electron microscope (SEM). Results: caffeine’s MIC was detected around 30 mg/mL, while the MFC was considered at 60 mg/mL. In an agar-well diffusion test, the inhibition zones were wider in caffeine groups. The Live/Dead viability test verified caffeine’s antifungal effects. The optical density of the adherent C. albicans on PMMA discs were lower at 620 nm or 410 nm in caffeine groups. CFU count was also reduced by caffeine treatments. SEM revealed the lower adherent C. albicans count in caffeine groups. The effect of caffeine was dose-dependent at which the 60 mg/mL dose demonstrated the most prominent effect. Conclusion: The study reinforced caffeine’s antifungal and antibiofilm properties and suggested it as an additive, or even an alternative, disinfectant solution for fungal biofilms on denture surfaces.

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Effects of Omeprazole and Caffeine Alone and in Combination with Gentamicin and Ciprofloxacin Against Antibiotic Resistant Staphylococcus Aureus and Escherichia Coli Strains

Cited by 30 publications

References 21 publications

Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

The Highest Dosage Combination Activity Screening from the Leaf Fraction of Melastoma malabathricum with Antibiotic Gentamicin and Ciprofloxacin

The Antifungal and Antibiofilm Activities of Caffeine against Candida albicans on Polymethyl Methacrylate Denture Base Material

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