Genetic differentiation and diversity upon genotype and phenotype in cowpea (Vigna unguiculata L. Walp.)

Xiong, Haizheng; Qin, Jun; Shi, Ainong; Mou, Beiquan; Wu, Dianxing; Sun, Jian; Shu, Xiaoli; Wang, Zhixue; Lu, Weiguo; Ma, Jianbing; Wang, Yuejin; Yang, Wei

doi:10.1007/s10681-017-2088-9

Cited by 20 publications

(18 citation statements)

References 66 publications

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“…As stated earlier, this lower level of variation among populations might be attributed to germplasm exchange among regions, limited introduction of new varieties to the farming system in each region and wider agro-ecological adaptation of the crop. This result is in agreement with many diversity studies in cowpea collections using different markers [30,48,67,74,85,86].…”

Section: Analysis Of Molecular Variancesupporting

confidence: 93%

DArTSeq SNP-based markers revealed high genetic diversity and structured population in Ethiopian cowpea [Vigna unguiculata (L.) Walp] germplasms

et al. 2020

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Cowpea [Vigna unguiculata (L.) Walp] is one of the important climate-resilient legume crops for food and nutrition security in sub-Saharan Africa. Ethiopia is believed to harbor high cowpea genetic diversity, but this has not yet been efficiently characterized and exploited in breeding. The objective of this study was to evaluate the extent and pattern of genetic diversity in 357 cowpea accestions comprising landraces (87%), breeding lines (11%) and released varieties (2%), using single nucleotide polymorphism markers. The overall gene diversity and heterozygosity were 0.28 and 0.12, respectively. The genetic diversity indices indicated substantial diversity in Ethiopian cowpea landraces. Analysis of molecular variance showed that most of the variation was within in the population (46%) and 44% between individuals, with only 10% of the variation being among populations. Model-based ancestry analysis, the phylogenetic tree, discriminant analysis of principal components and principal coordinate analysis classified the 357 genotypes into three well-differentiated genetic populations. Genotypes from the same region grouped into different clusters, while others from different regions fell into the same cluster. This indicates that differences in regions of origin may not be the main driver determining the genetic diversity in cowpea in Ethiopia. Therefore, differences in sources of origin, as currently distributed in Ethiopia, should not necessarily be used as indices of genetic diversity. Choice of parental lines should rather be based on a systematic assessment of genetic diversity in a specific population. The study also suggested 94 accesstions as core collection which retained 100% of the genetic diversity from the entire collection. This core set represents 26% of the entire collection pinpointing a wide distribution of the diversity within the ethiopian landraces. The outcome of this study provided new insights into the genetic diversity and population structure in Ethiopian cowpea genetic resources for designing effective collection and conservation strategies for efficient utilization in breeding.

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Section: Analysis Of Molecular Variancesupporting

confidence: 93%

DArTSeq SNP-based markers revealed high genetic diversity and structured population in Ethiopian cowpea [Vigna unguiculata (L.) Walp] germplasms

et al. 2020

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“…Domesticated cowpea in both West and East Africa shows relatively high levels of diversity compared with other populations worldwide, leading to competing proposals as to whether cowpea was domesticated in West (Ba, Pasquet, & Gepts, 2004) or East Africa (Xiong et al, 2018). Earlier studies using amplified fragment length polymorphism (AFLP; Coulibaly et al, 2002) and random amplification of polymorphic DNA (RAPD) markers (Ba et al, 2004) support domestication in West Africa despite noting that the Vigna genus is believed to have evolved in East Africa.…”

Section: Resultsmentioning

confidence: 99%

Genetic, textual, and archeological evidence of the historical global spread of cowpea (Vigna unguiculata[L.] Walp.)

Herniter

Muñoz‐Amatriaín

2020

Legume Science

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Cowpea (Vigna unguiculata [L.] Walp.) was originally domesticated in sub‐Saharan Africa but is now cultivated on every continent except Antarctica. Utilizing archeological, textual, and genetic resources, the spread of cultivated cowpea has been reconstructed. Cowpea was domesticated in Africa, likely in both West and East Africa, before 2500 BCE and by 400 BCE was long established in all the modern major production regions of the Old World, including sub‐Saharan Africa, the Mediterranean Basin, India, and Southeast Asia. Further spread occurred as part of the Columbian Exchange, which brought African germplasm to the Caribbean, the southeastern United States, and South America and Mediterranean germplasm to Cuba, the southwestern United States, and Northwest Mexico.

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“…Cowpea can grow in a wide range of environments including Asia, Africa, Central and South America, the United States, and parts of southern Europe. Genetic variation and environmental adaption of the species are highly associated with the evolution of the species; in crops this is influenced by human activities included domestication, cultivation, and immigration (Xiong et al, 2018). Despite the significant difference in seed protein content among cowpea varieties, some studies have reported genetic control of protein accumulation by three to seven genes (Fernandes et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular characterization of cowpea landraces using seed storage proteins and SRAP marker patterns

Alghamdi

Khan

Migdadi

et al. 2019

Saudi Journal of Biological Sciences

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Seven landraces of cowpea [Vigna unguiculata (L.) Walp.] were assessed for genetic variability in total proteins, protein fractions viz. albumins, globulins, prolamins, and glutelins by SDS-polyacrylamide gel electrophoresis and DNA polymorphism using sequence-related amplified polymorphisms (SRAP) markers. The solubility-based protein fractionation data indicated that the salt soluble fraction (globulin) and water-soluble fraction (albumin) proteins were the predominant fractions in cowpea seeds comprising 45–50.3% and 31.2–35.5% of total soluble proteins, respectively. The electrophoretic pattern revealed the molecular heterogeneity among total proteins as well as different protein fractions. The molecular weights of protein bands obtained by SDS-PAGE varied between 10 to 250, 15 to 110, 15 to 150, and 15 to 130 kDa for total proteins, albumins, globulins, and glutelins, respectively. A large number of bands were found common to the various landraces, indicative of their close relationship with one another. However, a few bands distinctive to some specific landraces were also detected, indicating varietal differences. A 34 SRAP primer pair combination generated a total of 1003 amplicons (loci) showed 100% polymorphism with an average of 0.93 polymorphism information content (PIC) value. Landraces displayed an average 0.50 similarity coefficient which clustered the landraces corresponding to their growth habit in main clusters and to their geographical origin in subcultures. Molecular and biochemical analysis were correlated with a medium level (Mantel test, r = 0.56, P < 0.02). These findings revealed that seed proteins and DNA polymorphism provide valuable information regarding the variability among landraces and this information could be utilized for breeding purposes in the enhancement of protein quality and quantity in grain legumes.

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Genetic differentiation and diversity upon genotype and phenotype in cowpea (Vigna unguiculata L. Walp.)

Cited by 20 publications

References 66 publications

DArTSeq SNP-based markers revealed high genetic diversity and structured population in Ethiopian cowpea [Vigna unguiculata (L.) Walp] germplasms

DArTSeq SNP-based markers revealed high genetic diversity and structured population in Ethiopian cowpea [Vigna unguiculata (L.) Walp] germplasms

Genetic, textual, and archeological evidence of the historical global spread of cowpea (Vigna unguiculata[L.] Walp.)

Biochemical and molecular characterization of cowpea landraces using seed storage proteins and SRAP marker patterns

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