2012
DOI: 10.31258/jnat.14.1.138-142
|View full text |Cite
|
Sign up to set email alerts
|

Optimalisasi Teknik Ekstraksi dan Isolasi DNA Tanaman Suren (Toona Sureni Merr.) untuk Analisis Keragaman Genetik berdasarkan Random Amplified Polymorphic DNA (RAPD)

Abstract: The species of trees have different secondary compounds that need optimum extraction techniques. Appropriate extraction techniques determine the quality and quantity of DNA produced. This research aims to found optimal of extraction methods and DNA isolation, then to created genome DNA in high quality and quantity, so that it can be using for genetic variation analyses in Suren (Toona sureni Merr.) by Random Amplified Polymorphic DNA (RAPD). This study shows that DNA concentrates were 763.3 μg/ml, 180.0 μg/ml,… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
5
0
4

Year Published

2018
2018
2021
2021

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 11 publications
(9 citation statements)
references
References 3 publications
0
5
0
4
Order By: Relevance
“…The thinness of the DNA band indicates the low of DNA concentration. This is due to that incomplete lysis in DNA extraction process, or some supernatant containing DNA genomic was wasted (Restu et al 2012). In addition, all DNA bands were smear, showing the poor DNA quality.…”
Section: Dna Extractionmentioning
confidence: 99%
“…The thinness of the DNA band indicates the low of DNA concentration. This is due to that incomplete lysis in DNA extraction process, or some supernatant containing DNA genomic was wasted (Restu et al 2012). In addition, all DNA bands were smear, showing the poor DNA quality.…”
Section: Dna Extractionmentioning
confidence: 99%
“…Conducted by method of Doyle and Doyle (1987) in Restu et al (2012) modified. A total of 200 mg of leafless rice leaves plus 0,02 g of PVP were crushed until smooth (flour).…”
Section: 3ctabmentioning
confidence: 99%
“…The genetic information of a plant variety can be known by the PCR technique. DNA isolation was the first step in molecular analysis of PCR (Restu et al, 2012). The purity of target DNA is very important in the PCR process, since impurities of DNA suspension can affect the amplification reaction and may inhibit the action of DNA polymerase enzymes.…”
Section: Introductionmentioning
confidence: 99%
“…Hasil penelitian ini, menunjukkan isolasi DNA ke-36 sampel tanaman berhasil dilakukan dengan metode CTAB dan buffer deterjen surfaktan ABS. Berdasarkan Tabel 2, ekstrak DNA daun resak (1c), kelat (5c), kulim (6c), ulin (7c), meranti (8c), marpoyan (10c, 10r) marsawa (11r) menunjukkan kualitas yang paling bagus hal ini disebabkan adanya optimalisasi suhu dan lama inkubasi yaitu sebesar 65°C selama 60 menit hal ini sesuai dengan penelitian sebelumnya yang dilakukan oleh Restu et al, (2012) Deterjen dengan surfaktan ABS ˃20% akan menyebabkan rusaknya membran sel melalui ikatan yang dibentuk melalui sisi hidrofobik deterjen dengan protein dan lemak pada membran sel sehingga akan membentuk senyawa "lipid protein-deterjen kompleks". Senyawa tersebut dapat terbentuk karena protein dan lipid memiliki ujung hidrofilik dan hidrofobik, demikian juga dengan deterjen, sehingga dapat membentuk suatu ikatan kimia.…”
Section: B Kualitas Hasil Isolasi Genomik Dnaunclassified