Harnelly E, Thomy Z, Fathiya N. 2018. Phylogenetic analysis of Dipterocarpaceae in Ketambe Research Station, GunungLeuser National Park (Sumatra, Indonesia) based on rbcL and matK genes. Biodiversitas 19: 1074-1080. Gunung Leuser National Parkhas several Research Stations. Ketambe is one of the Research Stations which is located in Aceh Tenggara district, Aceh province. Oneof the timber plants family which is abundant in Leuser Mountain is Dipterocarpaceae. However, the data of species and relationship ofDipterocarpaceae in Ketambe Research Station is lack and not available. The aim of the study is to analyze the phylogenetic ofDipterocarpaceae based on rbcL and matK gene in Ketambe Research Station. This research was carried out from July 2015 to August2016 in Ketambe Research Station and Forestry and Forest Genetics Laboratory of Molecular, Bogor Agricultural University. Themethod used quadrat sampling technique with purposive sampling and experimental laboratory consisting of DNA extraction, PCR,electrophoresis, and sequencing. The data analysis was done using BioEdit and MEGA6. The results showed that based onmorphological identification, there were five Dipterocarpaceae species found namely; Parashorea lucida, Shorea parvifolia, Shorealepidota, Shorea johorensis, and Hopea dryobalanoides. The phylogenetic tree based on rbcL gene showed that there were twomonophyletic groups, the first group was S. johorensis, S. lepidota, and H. dryobalanoides; and the second group consisted of S.parvifolia and P. lucida. The phylogenetic tree reconstruction based on matK gene showed that Shorea parvifolia and S. johorensis wereseparated in two different monophyletic groups.