Quality transitivity of Danhong Huayu Koufuye: A study on chemical profiles of medicinal herbs, compound preparation and dosed rat plasma using ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Abstract

Danhong Huayu Koufuye (DHK), an effective Chinese medicine preparation, is mainly used for the treatment of blurred vision and sudden blindness caused by qi stagnation and blood stasis, as well as the absorption period of central retinal vein occlusion. However, the current quality standard is relatively low, only stipulating the content of protocatechualdehyde. Chemical transitivity is the basis for discovering quality markers and is used in quality process control of Chinese medicines. Herein, the chemical profiles of seven medicinal herbs, DHK and dosed rat plasma were comprehensively analyzed using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. As a result, 134 chemical constituents were identified in seven medicinal herbs, including salvianolic acids, diterpene quinones, phenolic acids, phthalides, cyanogenic glycosides, flavonoids and triterpenoid saponins.Among them, 55 chemical constituents were transferred to DHK along with extraction and preparation, and 26 were further absorbed into blood and metabolized to produce 11 metabolites after oral administration. The transitivity of DHK from medicinal herbs to compound preparation and into blood was analyzed for the first time. This article will be valuable to ascertain the quality markers for quality process control and further pharmacokinetic studies. K E Y W O R D Schemical profiles, Danhong Huayu Koufuye, mass spectrometry, quality transitivity, ultraperformance liquid chromatography

Comprehensive characterization of multiple components ofZiziphus jujuba MillusingUHPLC‐Q‐ExactiveOrbitrap Mass Spectrometers

“…Compound 10, eluted at 1.37 min, exhibited a quasi‐molecular ion at [M‐H] − m/z 490.0981, generated the main fragment ion at m/z 328.0432 by the loss of a glucose group (C 6 H 10 O 5 , 162 Da), and was identified as CAMP‐O‐glucoside. Compounds 1, 3, and 5, found at 0.81, 0.89, and 1.08 min, respectively, possessed the same quasi‐molecular ion at [M‐H] − m/z 341.1089 and were tentatively identified as sucrose isomers based on a previous study (Zhang et al, 2020 ).…”

“…Compounds 2 and 6 were eluted at 0.86 and 1.14 min, respectively, with a quasi‐molecular ion [M‐H] − at m/z 191.0197 (C 6 H 8 O 7 ) and the fragment ion at m/z 111.0073, suggesting that they were citric acid isomers (Zhang et al, 2020 ). Similarly, compounds 11 and 13 were tentatively assigned to 3‐methylglutaric acid isomers (Bruderer et al, 2017 ).…”

“…Compounds 16, 27, 32, 37, 38, 51, and and the fragment ion at m/z 111.0073, suggesting that they were citric acid isomers (Zhang et al, 2020). Similarly, compounds 11 and 13…”

“…Compounds 26,31,39,42,47,49,118,146,149,194,and 256 were well characterized according to their MS and MS/MS spectra (Jung et al, 2019;Meng et al, 2020;Ostrowski et al, 2014;Ricciutelli et al, 2019;Tóth et al, 2015). as sucrose isomers based on a previous study (Zhang et al, 2020).…”