Regeneration and micropropagation of lemon cultivars in vitro from nodal explants

Самарина, Л. С.; Kolomiets, T. M.; Баранова, Е. Н.; Arutyunova, E. S.

doi:10.3103/s106836741006008x

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Hal ini juga terjadi pada perlakuan A1 media M129, sehingga dapat dikatakan parameter panjang akar lebih dipengaruhi oleh penambahan karbon aktif dan gula secara tunggal maupun kombinasinya (Tabel 2). Selain itu hasil penelitian Samarina et al (2010), penggunaan GA yang dikombinasikan dengan NAA dan 3 BAP konsentrasi rendah mampu meningkatkan morfogenesis lemon, kombinasi NAA dan GA pada 3 kultur embrio kelapa sawit Dura mampu berperan dalam meningkatkan inisiasi tunas dan regenerasinya (Suranthran et al, 2011). Oleh karena itu, perlakuan A6 yaitu penambahan GA +NAA tanpa penambahan karbon 3 aktif memiliki persen perkecambahan tinggi namun proses perkecambahannya mengalami waktu yang lambat jika dibandingkan dengan keempat perlakuan lainnya yang mulai bertunas pada minggu ke-4 dan berakar pada minggu ke-7 (Tabel 1 dan Gambar 3).…”

Section: Pengaruh Penambahan Zpt Karbon Aktif Dan Sukrosa Pada Mediunclassified

THE ROLE OF NAA, GA3, ACTIVATED CHARCOAL, AND SUCROSE ON ZYGOTIC EMBRYOS CULTURE OF HYBRID OG OPEN POLLINATED CLONE (Elaeis guineensis JACQ. X Elaeis oleifera)

Ernayunita

Rahmadi

Harahap

et al. 2018

J. Pen. Kelapa Sawit

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Oil palm OG hybrid genetic potentials are high in term of vegetative and generative traits, such as: low height increment, compact palm, high free fatty acid and high carotenes. However, OG hybrid reproduction is hindered due to its low germination rate. So, alternative method for germination is needed, which is in vitro embryo zygotic culture. This study objective was to reveal the best medium composition for OG hybrid embryo culture by modificated several oil palm basic medium from oil palm tissue culture protocol and to understand the role of plant growth regulators, activated charcoal, and sucrose on in vitro growth and development of OG hybrid embryo. The design used was Completely Randomized Design (CRD) with 16 replication and 4 embryos for each replicates, while the treatments were: A1 = M129 (control); A2 = M129 + activated charcoal; A3 = M129+ activated charcoal + sucrose; A4 = ½ M129 + activated charcoal + sucrose; A5 = M129 + GA3 ; and A6 = M129 + GA3 + NAA. From the study we found that the best medium for OG hybrid open pollinated clone's embryo culture was M129 with GA3 and NAA (A6). This medium gave percentage of in vitro germination up to 14.06%, and later on the plantlets from this medium gave the best vegetative growth performance such as high number of leaves and primer roots.

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Section: Pengaruh Penambahan Zpt Karbon Aktif Dan Sukrosa Pada Mediunclassified

THE ROLE OF NAA, GA3, ACTIVATED CHARCOAL, AND SUCROSE ON ZYGOTIC EMBRYOS CULTURE OF HYBRID OG OPEN POLLINATED CLONE (Elaeis guineensis JACQ. X Elaeis oleifera)

Ernayunita

Rahmadi

Harahap

et al. 2018

J. Pen. Kelapa Sawit

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Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants

Singh

Kaur

2011

J. Crop Sci. Biotechnol.

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Regeneration and micropropagation of Meyer lemon (Citrus x meyeri) supported by polymorphism analysis via molecular markers

Haradzi

Khor

Subramaniam

et al. 2021

Scientia Horticulturae

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Regeneration and micropropagation of lemon cultivars in vitro from nodal explants

Cited by 4 publications

References 6 publications

THE ROLE OF NAA, GA3, ACTIVATED CHARCOAL, AND SUCROSE ON ZYGOTIC EMBRYOS CULTURE OF HYBRID OG OPEN POLLINATED CLONE (Elaeis guineensis JACQ. X Elaeis oleifera)

THE ROLE OF NAA, GA3, ACTIVATED CHARCOAL, AND SUCROSE ON ZYGOTIC EMBRYOS CULTURE OF HYBRID OG OPEN POLLINATED CLONE (Elaeis guineensis JACQ. X Elaeis oleifera)

Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants

Regeneration and micropropagation of Meyer lemon (Citrus x meyeri) supported by polymorphism analysis via molecular markers

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