The effect of adipose tissue derived MSCs delivered by a chemically defined carrier on full-thickness cutaneous wound healing

Jiang, Dongsheng; Qi, Yu; Walker, Nathan G.; Sindrilaru, Anca; Hainzl, Adelheid; Wlaschek, Meinhard; MacNeil, Sheila; Scharffetter‐­Kochanek, Karin

doi:10.1016/j.biomaterials.2012.12.014

Cited by 99 publications

(84 citation statements)

References 54 publications

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“…In this study, we observed a greater amount of newly formed vessels in the group containing ASCs as compared with the other groups tested. These results are in accordance with other studies that demonstrate the paracrine effect exercised by mesenchymal stem cells during wound healing (41,43).…”

Section: Discussionsupporting

confidence: 95%

“…It is also known that mesenchymal stem cells produce growth factors such as VEFG and β-FGF that stimulate the neo-vascularization (40)(41)(42) process required for promoting wound maturation and healing (43). In this study, we observed a greater amount of newly formed vessels in the group containing ASCs as compared with the other groups tested.…”

Section: Discussionmentioning

confidence: 59%

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Bilaminar Device of Poly(Lactic‐co‐Glycolic Acid)/Collagen Cultured With Adipose‐Derived Stem Cells for Dermal Regeneration

et al. 2016

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Several materials are commercially available as substitutes for skin. However, new strategies are needed to improve the treatment of skin wounds. In this study, we developed and characterized a new device consisting of poly(lactic-co-glycolic acid) (PLGA) and collagen associated with mesenchymal stem cells derived from human adipose tissue. To develop the bilaminar device, we initially obtained a membrane of PLGA by dissolving the copolymer in chloroform and then produced a collagen type I scaffold by freeze-drying. The materials were characterized physically by gel permeation chromatography, scanning electron microscopy, and mass loss. Biological activity was assessed by cell proliferation assay. A preliminary study in vivo was performed with a pig model in which tissue regeneration was assessed macroscopically and histologically, the commercial device Integra being used as a control. The PLGA/collagen bilaminar material was porous, hydrolytically degradable, and compatible with skin growth. The polymer complex allowed cell adhesion and proliferation, making it a potentially useful cell carrier. In addition, the transparency of the material allowed monitoring of the lesion when the dressings were changed. Xenogeneic mesenchymal cells cultured on the device (PLGA/collagen/ASC) showed a reduced granulomatous reaction to bovine collagen, down-regulation of α-SMA, enhancement in the number of neoformed blood vessels, and collagen organization as compared with normal skin; the device was superior to other materials tested (PLGA/collagen and Integra) in its ability to stimulate the formation of new cutaneous tissue.

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Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 59%

Bilaminar Device of Poly(Lactic‐co‐Glycolic Acid)/Collagen Cultured With Adipose‐Derived Stem Cells for Dermal Regeneration

et al. 2016

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“…Adipose-derived stromal cells have been reported to increase the rate of re-epithelialization and blood vessel density in skin wounds (Alexaki et al 2012;Forcheron et al 2012;Huang et al 2012;Riccobono et al 2012). Additionally, transplantation of adiposederived stromal cells into wound beds has been reported to change the phenotype of wound bed macrophages from pro-to anti-inflammatory (Ziboh et al 1986;Jiang et al 2013). Because mesenchymal cells can produce both fibroblast and adipocyte fates in vitro (Uezumi et al 2011), it will be interesting to determine whether transplanted mesenchymal cells generate adipocyte lineage cells to enhance the wound healing process.…”

Section: Adipocytes During Skin Wound Healingmentioning

confidence: 99%

Adipocytes in Skin Health and Disease

Rivera-Gonzalez

Shook

Horsley

2014

Cold Spring Harbor Perspectives in Medicine

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Adipocytes are intimately associated with the dermal compartment of the skin, existing in a specialized dermal depot and displaying dynamic changes in size during tissue homeostasis. However, the roles of adipocytes in cutaneous biology and disease are not well understood. Traditionally, adipocytes within tissues were thought to act as reservoirs of energy, as thermal, or as structural support. In this review, we discuss recent studies revealing the cellular basis of the dynamic development and regenerative capacity of dermal adipocytes associated with the hair cycle and following injury. We discuss and speculate on potential roles of dermal adipocytes in cutaneous biology with an emphasis on communication during hair follicle growth and wound healing. Finally, we explore how alterations in the dermal adipose tissue may support clinical manifestations of cutaneous diseases such as lipodystrophy, obesity, and alopecia. ADIPOCYTE DEVELOPMENT AND HOMEOSTASIS IN THE SKIN

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“…MSCs mechanisms that improve HF formation are, however, still elusive. 45 The identification of transplanted MSCs in the neoepidermis 27,42,46 has been supporting the hypothesis of transdifferentiation into epidermal cells. However, despite the set of in vitro studies, [47][48][49] this hypothesis is still poorly explored.…”

Section: Building Blocksmentioning

confidence: 82%

“…Considering the different number of works using stem cells either injected at the wound borders 15,36 or randomly incorporated within biomaterial-based matrices, [37][38][39][40][41][42] there is skepticism regarding the need for 3D organization. Nonetheless, this discrepancy can be correlated with the limitations of MSC differentiation toward epithelial and endothelial lineages, 43 as well as to the deficient purity/stability of epidermal stem cells cultures.…”

Section: Building Blocksmentioning

confidence: 99%