Abstract. Pratama AM, Herawati O, Nuranisa NR, Hanifah N, Wijayanti AD, Rahmatullah S, Nurani E, Budiyanto A. 2021. Identification of poisonous plants and their solutions for traditional livestock in Bojonegoro District, East Java, Indonesia. Biodiversitas 23: 446-452. Local farmers in Bojonegoro District use plants as the main food source and to treat several diseases for their livestock. However, they also comprise a large variety of poisonous plants known by a secondary chemical compound that causes death in the livestock. This study aims to identify the potentially poisonous plants to livestock and their veterinary importance. The study was conducted by interviewing local farmers as we require data as a source of information to provide an overview of their knowledge and experience about poisonous plants that can endanger livestock health. Respondents consisted of 40 local farmers from more than 20 livestock groups in Bojonegoro District. Data were analyzed by Frequency of Citation (Fic), Relative Frequency of Citation (RFC), Frequency of Plant Part (FPP), Symptoms of Poisonous Plants Frequency (SPPF), Livestock Affected Frequency (LAF). A total of nine plants were identified and documented to have poisonous effects on livestock animals, namely leaf of lophatheri (Lophatherum gracile), leaf and tuber skin of cassava (Manihot esculenta), leaf of jack bean (Canavalia ensiformis), leaf of sorghum (Sorghum bicolor), leaf of Chinese albizia (Albizia chinensis), leaf of sweet potato (Ipomoea batatas), leaf of peanut (Arachis hypogaea), rotten fruit of (jackfruit Artocarpus heterophyllus), and leaf of winged bean (Psophocarpus tetragonolobus). Leaf of Lophatherum gracile was the most poisonous plant part for livestock. Furthermore, the leaves of plants are frequently poisonous. Bloating, hypersalivation, frothy mouth, death, and inappetence were among the frequently manifested signs by poisoned livestock. Moreover, this study showed that ruminants species were the most susceptible to poisoned animals. Improper handling of plants for animal feed is thought to trigger poisoning. Therefore a proper feeding preparation is needed before being given as forage. Although those plants can cause toxic effects on livestock, they have tremendous potential to become herbal medicine with the proper formulation.
Abstract. Pratama AM, Herawati O, Nabila AN, Belinda TA, Wijayanti AD. 2021. Ethnoveterinary study of medicinal plants used for cattle treatment in Bojonegoro District, East Java, Indonesia. Biodiversitas 22: 4236-4245. Bojonegoro is a rural district in Indonesia's East Java Province where farming and cattle rearing are the main economic activities. The Bojonegoro District's cattle producers employ some medicinal plants specifically for the treatment of bovine illnesses. However, no data has been reported thus far. The goal of this research was to find and document ethnoveterinary medicinal herbs for cattle cures in the Bojonegoro District. A total of 41 cattle breeders were interviewed for the study. To collect demographic and ethnoveterinary medicinal plant data, each informant was interviewed using a semi-structured questionnaire in the native language of each informant. The stastitical analysis in this study include informant consensus (Fic), Fidelity Level (FL), and Plant Part Frequency (PPF). Approximately 78.00% of the respondents are between the ages of 30 and 50, with 36.59% having only graduated from senior high school. The Peranakan Ongole (PO) is the most common breed preserved by cattle breeders. In the study area, 41 ethnoveterinary medicinal plants were mentioned by male respondents to cure cattle health problems. Digestive illnesses are the most frequent ailments in cattle treated with medicinal plants. Curcuma longa L. was the most commonly mentioned medicinal plant. A majority of the source ethnoveterinary medicinal plants were cultivated on-site and the leaves of these plants were most often used.
Background and Aim: The utilization of cassava leaves and peels, ceara rubber leaves, sweet potato leaves, Chinese Albizia leaves, and lophatheri leaves from Bojonegoro Regency has led to the poisoning of livestock due to antinutritional factors. Nevertheless, the plants are known to have bioactive components and potential antioxidant and antibacterial activity if appropriately processed. This study aimed to determine the antinutritional compounds as well as the antioxidant and antibacterial potential of these plants responsible for livestock poisoning in the Bojonegoro Regency. Materials and Methods: Extraction was performed by the maceration method using 70% (v/v) ethanol solvent. The samples were analyzed qualitatively to determine the presence of tannins, alkaloids, oxalates, cardiac glycosides, and cyanogenic glycosides. The antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl method, while the antimicrobial activity was assessed by different testing concentrations (125, 250, and 500 mg/mL) against Staphylococcus epidermidis, Staphylococcus aureus, and Escherichia coli. Results: The ethanolic extract of the plants was found to contain antinutritional tannins, alkaloids, cardiac glycosides, and cyanogenic glycosides suspected of causing livestock poisoning. Despite the presence of these antinutrients, all extracts also had antioxidant and antibacterial potential. Cassava peels and sweet potato leaves had the highest antioxidant activity, whereas Chinese Albizia leaves had the most potent antibacterial activity. Conclusion: Cassava leaves and peels, ceara rubber leaves, sweet potato leaves, Chinese Albizia leaves, and lophatheri leaves obtained from Bojonegoro Regency and used as agricultural waste contain antinutritional factors but also possess potentially effective antioxidant and antimicrobial components.
Background and Aim: Enrofloxacin and tylosin can be combined into an antibiotic formulation which is expected to have a broader range of antibacterial activity against various infections in broilers. Validation method analysis of the levels of these two active compounds needs to be done for future use in pharmacokinetic or residual studies. The present study aims to determine a suitable validation method of isocratic high-performance liquid chromatography (HPLC) to measure the concentration of antibiotic combinations in the broiler liver, kidney, and muscles. Materials and Methods: The combination of enrofloxacin and tylosin in the liver, kidney, and muscle was validated by HPLC method to find the procedures, processes, equipment, and systems used, consistently provides the appropriate results. The chromatography system consisted of an Octadecyl-silica column of 5 μm in diameter and 150 mm in length with a mobile phase of a mixture of 0.05 M monobasic sodium phosphate (pH 2.5) and acetonitrile (65:35 v/v). The solution was detected at a wavelength of 280 nm, 30°C, a flow rate of 1 mL/min, and an injection volume of 20 μL. The combination antibiotics powder was produced from PT Tekad Mandiri Citra, Bandung, Indonesia, and broiler tissues obtained from day-old chick broilers maintained for 30 days with free antibiotic feed. Results: Validation of a combination solution of enrofloxacin and tylosin shows the linearity values of enrofloxacin and tylosin in the liver, kidney, and muscles as r2=0.9988, r2=0.9999, r2=0.9997, r2=0.9989, r2=0.9978, and r2=0.9962. The accuracy and precision values of enrofloxacin in the liver, kidney, and muscles were 5.53, 6.23, and 6.93, respectively. The values of accuracy and precision of tylosin in the liver, kidney, and muscles were 10.43, 4.63, and 7.16%, respectively. The retention times for enrofloxacin and tylosin were 1.945-2.000 min and 4.175-4.342 min. The limit of detection (LOD) and limit of quantity (LOQ) values for enrofloxacin were 3.03 and 10.1 μg/g, respectively. In contrast, the LOD and LOQ values for tylosin were 9.05 and 30.17 μg/g, respectively. Conclusion: The value of linearity, accuracy, precision, specificity, and sensitivity of the combined solution of enrofloxacin and tylosin showed promising results.
Abstract. Terminal Type A Bandar Raya Payung Sekaki is the largest terminal in the city of Pekanbaru and Riau Province with quite volatile arrival and departure movements. The current condition of the BRPS Terminal is not as expected because there are still many passengers who are reluctant to get on and off at the terminal because the location of the terminal is quite far from the center of activities such as the center of trade, socio-economic and other activities so that there is a lack of movement through the BRPS Terminal. The analytical method used is qualitative and quantitative analysis. Qualitative analysis is used to describe current conditions such as system activity conditions, terminal utilization, terminal accessibility and SWOT. While the quantitative analysis is IPA analysis, movement and passenger potential analysis using growth factors and terminal income potential analysis. From the results of the SWOT analysis, it is stated that the terminal is in quadrant I (aggressive). From the results of the IPA, there are 15 service indicators that need to be improved. From the analysis of potential demand, there are 25.34% additional passengers for AKDP and 41.61% for AKAP. From the results of the analysis of potential income has a potential income of Rp. 569,250,000/year of potential land lease. Based on the analysis of potential demand and income, the terminal has great potential to be optimized. Terminal optimization is carried out by improving terminal services, increasing terminal accessibility and connectivity Abstrak. Terminal Tipe A Bandar Raya Payung Sekaki merupakan terminal terbesar di kota Pekanbaru dan Provinsi Riau dengan pergerakan kedatangan dan keberangkatan yang cukup fluktuatif. Kondisi Terminal BRPS saat ini tidak seperti yang diharapkan karena masih banyak penumpang yang enggan naik dan turun di terminal karena lokasi terminal berada cukup jauh dari pusat kegiatan seperti pusat perdagangan, sosial ekonomi dan kegiatan lainnya sehingga kurangnya pergerakan yang melewati Terminal BRPS. Metode analisis yang digunakan adalah analisis kualitatif dan kuantitatif. Analisis kualitatif digunakan untuk menggambarkan kondisi terkini seperti kondisi sistem aktivitas, pemanfaatan terminal, aksesibilitas terminal dan SWOT. Sedangkan analisis kuantitatif yaitu analisis IPA, analisis potensi pergerakan dan penumpang menggunakan growth factor dan analisis potensi pendapatan terminal. Dari hasil analisis SWOT menyatakan terminal berada di kuadran I (agresif). Dari hasil IPA terdapat 15 indikator pelayanan yang perlu ditingkatkan. Dari hasil analisis potensi demand terdapat penambahan penumpang AKDP 25,34% dan AKAP 41,61%. Dari hasil analisis potensi pendapatan memiliki pendapatan potensial sebesar Rp. 569.250.000/tahun dari sewa lahan potensial. Berdasarkan hasil analisis potensi demand dan pendapatan maka terminal memiliki potensi yang besar untuk dioptimalkan. Optimalisasi terminal dilakukan dengan cara meningkatan pelayanan terminal, meningkatan aksesibilitas dan konektivitas terminal
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