Rasulullah Shallahu'alaihiwassalam said, "if a fly falls in the vessel anyone of you, let him dip all of it (in the vessel) and then throw it away, for in one of its wings has the ailment and the other has the cure" (Al-Bukhari). This hadith creates controversy because in general flies are a vector for the spread of disease from dirty places to food or drinks. Therefore, the research was conducted on right-wing of fly (Musca domestica) as neutralization of drinks contaminated by a microbe. This research used the method of Complete Random Design by 5 treatments and 2 repetitions. The treatment was done by sterilized water (positive control), drinking water added to the bacteria Escherichia coli (negative control), and drinking water contaminated by Escherichia coli bacteria with the addition of 1, 2, and 3 flies right-wings. The research began with taking the right-wing of fly and continued with the dilution of Escherichia coli culture tubes up to 6 times. The bacterial culture inoculation was carried out using Pour Plate method on Eosin Methylene Blue agar which is incubated for 12-48 h at a temperature of 37˚C. Data retrieval is done by observing the calculation of the number of microbes using a colony counter every 12 h. The data which obtained for 48 h incubation show "0" as the result, that cannot be analyzed with SPSS. The result indicates the microbial development does not occur on contaminated drinks by addition with right-wing of Musca domestica.

The second-hand smoker will have difficulties because of nicotine and other substances leading to activate free radical in the body and stimulate the secretion of inflammatory mediators. Flavonoid is a compound contained in marigold leaves, believed to have anti-inflammatory effects. This study aims to look at the effect of marigold leaves to neutrophil and lymphocyte profile of Wistar rat exposed cigarette smoked. This research was an experimental laboratory research design with pre and post-control groups. The sample consisted of 30 male Wistar rats. The control group was given food and drink in an ad libitum without given marigold, while treatments group 1 and 2 were given marigold at a dose of 4.5 and 6.3 grams, respectively. Each group was exposed to 2 cigarettes smoked per day for 2 weeks. On days 0, 7, and 14, blood samples were taken through retro orbitals. The data were analyzed by using the Sysmax KX-21® Hematology Analyzer. Normality test used Shapiro-wilk continued to one way ANOVA test and finally DUNCAN test. One way ANOVA test results for neutrophils and lymphocytes stated p = 0.00, which means that the effect of giving marigold to levels of neutrophils and lymphocytes of Wistar rats exposed cigarette smoked, the DUNCAN test results stated the dosage of 4.5 and 6.3 not significantly different but gave a significant difference to the control group. Marigold significant to reduce levels of neutrophils and lymphocytes to the normal stage of wistar rats exposed cigarette smoked.

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