Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.
Lidah mertua (Sansevieria trifasciata Prain) merupakan tanaman yang berasal dariAfrika dan dikenal sebagai antimikroba, serta berkhasiat obat. Tujuan dari penelitian iniialah mengetahui kandungan antimikroba ekstrak daun lidah mertua dengan menentukanlebar daerah hambat (LDH) terhadap khamir Candida albicans menggunakan metode difusikertas cakram. Pengujian LDH dilakukan terhadap konsentrasi ekstrak daun lidah mertua60%, 70% , 80%, 90%, serta ketokonazol 14 ppm sebagai kontrol positif dan karboksi metilselulosa (CMC) 0,5% sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrakdaun lidah mertua pada konsentrasi 90% membentuk zona hambat terhadap C. albicansyang paling luas tetapi tidak jernih. Hasil analisis mutu ekstrak diketahui bahwa kadar abu ekstrak daun lidah mertua yang tidak larut dalam asam ialah 0,23% dan yang larut dalamair ialah 5,04%. Sedangkan hasil penetapan kadar sari ekstrak daun lidah mertua yang larutdalam air ialah 38,76% dan yang larut dalam etanol ialah 12,53%. Hasil fitokimia diketahui ekstrak daun lidah mertua mengandung saponin, flavonoid, steroid, dan triterpenoid, yangberfungsi dapat menghambat C. albicans.Kata kunci : daun lidah mertua (Sansevieria trifasciata Prain), Candida albicans, efektivitas,antikhamir
Waste treatment technology is not less important for the business of Slaughterhouse (RPH). Various businesses and technologies have been done to handle this waste problem, especially for the rumen waste in RPH. One of them is utilizing the waste as an organic fertilizer. Bovine rumen bacteria are bacteria that originate from the contents of bovine rumen, with simple and inexpensive development techniques. This bacterium helps farmers in improving and developing an insightful agriculture on the preservation of soil fertility and natural resources. The study aims to identify bacteria and find out the diversity, properties, and characteristics of bacteria from the liquid organic content of rumen cows. Methods undertaken by observing colony morphology, colony selection and purification, Gram staining, spores staining, and biochemical testing. Of the four isolates identified there are two bacterial genus, three isolates RS1a, RS2a, RS2b including the genus Bacillus sp. aerobic and an isolates of RS1b including the genus Lactobacillus sp. the facultative of anaerobic. The four isolates of bacteria are able to grow optimum at pH 6.5 and the temperature of 370C is homofermentative.
Penelitian efektivitas antibakteri ekstrak etanol dan ekstrak air kelopak bunga rosella terhadap Streptococcus pneumoniae telah dilakukan menggunakan metode difusicakram dan dilusi secara invitro. Larutan induk ekstrak etanol dan ekstrak air rosella70g/100 ml diencerkan dengan konsentrasi 10%, 30%, 50% dan70% untuk metodedifusi cakram dengan kontrol positif ampisilin 10 IU, dan ekstrak etanol diencerkandengan konsentrasi 0,1%, 0,25%, 0,5%, 1%, dan 2% untuk metode dilusi terhadapS.pneumoniae. Hasil penelitian menunjukkan bahwa ekstrak etanol dan ekstrak airrosella dapat menghambat pertumbuhan bakteri S.pneumoniae diketahui dari zonahambat yang terbentuk. Konsentrasi 70% pada metode difusi ekstrak etanol danekstrak air rosella membentuk diameter zona hambat paling luas rata-rata 25,6 mmuntuk ekstrak etanol dan 24,3 mm untuk ekstrak air rosella. Hasil konsentrasi hambatminimum dari ekstrak etanol rosella yang paling efektif adalah konsentrasi 1%. Hasilpengujian fitokimia diketahui bahwa ekstrak etanol dan ekstrak air rosellamengandung saponin, tanin, dan flavonoid.Kata kunci : Kelopak Bunga Rosella (Hibiscus sabdariffa L), S.pneumoniae, antibakteri,ekstrak etanol, ekstrak air
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.