Amélioration par fractionnement chromatographique de l’activité anticandidosique d’un extrait hexanique de Mitracarpus scaber Zucc. sur la croissance in vitro de Candida albicans et Candida tropicalis

Abstract

Dans le but d'apporter notre contribution à la lutte contre les candidoses opportunistes, nous avons testé dix-sept fractions chromatographiques d'un extrait hexanique de Mitracarpus scaber codifié Misca sur la croissance in vitro de Candida albicans et Candida glabrata. Les tests antifongiques ont été réalisés sur milieu Sabouraud auquel l'extrait végétal a été incorporé selon la méthode de la double dilution en tubes penchés. Les concentrations varient de 3 125 à 12 µg/ml. Les résultats ont montré que dans cet intervalle de valeurs, seules les fractions F 6 , F 7 , F 8 ont permis une nette inhibition de la croissance des deux souches de Candida à 781 µg/ml. Parmi elles, la fraction F 8 possède la meilleure activité anticandidosique avec une CI 50 = 16 µg/ml sur Candida albicans contre CI 50 = 45 µg/ml sur Candida tropicalis qui est donc la souche la moins sensible. La fraction F 8 de l'extrait hexanique est donc celle qui concentre le mieux les principes actifs anticandidosiques de Misca. Mots clés : Misca -Activité anticandidosique -Fractionnement chromatographique Improvement by chromatographic dividing of anticandidosic activity of an hexanic extract Mitracarpus scaber on the in vitro growth of Candida albicans and Candida tropicalisAbstract: In the goal to bring our contribution to the struggle against opportunistic candidosis, we tested seventeen chromatographic fraction gotten from an hexanic extract of a Mitracarpus scaber Misca codified on the in vitro growth of Candida albicans and Candida tropicalis. Antifungal tests have been made on Sabouraud medium culture. Extract has been inserted to this medium according to the Agar slant method. The range of concentrations has been fixed between 3.125 µg/ml and 12 µg/ml. Results showed that in this interval of values, alone fractions F 6 , F 7 , F 8 permitted a clean inhibition to 3 125 µg/ml of the growth of the 2 strains of Candida. Among them, the fraction F 8 has possessed a better anticandidosic activity with IC 50 = 16 µg/ml on Candida albicans against IC 50 = 45 µg/mls on Candida tropicalis which is the least sensible. So, the chromatographic fraction F 8 obtained from a hexanic extract is the one that permits a better concentration of active principles of Misca.

Antifungal activities of medicinal plants extracts of Ivorian pharmacopoeia

“…This shows that for all plant species, hydro-ethanolic extracts concentrate a greater proportion of active principles than their aqueous equivalents. This assertion is similar to former investigation reports [ 2 , 13 , 15 , 16 , 29 - 31 , 33 ]. Indeed, the results of these previous investigations revealed that all the hydro-ethanolic extracts tested were 2-16 times more active than the equivalent aqueous extracts.…”

Antifungal potential of improved crude extracts of Mitracarpus scaber (Zucc) against Candida guilliermondii and Candida parapsilosis

“…6,[13][14][15] Antifungal activity has been evaluated on C. albicans, C. glabrata and C. tropicalis in 2009 and 2010. [16][17][18][19] This study completes data on anticandidosic spectrum of organic extracts of this plant in comparison to ketoconazole, a standard antifungal. To achieve this general objective, the following specific objectives are defined: to prepare vegetable extracts and to assess antifungal activity of these extracts.…”

“…Extract X 0 was concentrated with a rotary evaporator of BÜCHI at 60°C. 18 To perform hydroalcoholic extract (X 0 ), this extract was used to prepare 6 other extracts:…”